Pusa e Ave'esea ai Su'i Nucleic (A02)
Fa'avae su'esu'ega
A maeʻa ona tuʻuina atu le DNA genomic e ala i le vaeluaina o sela ma le lysis buffer, e mafai e le pulu mageta ona filifili filifili i le DNA genomic i le faʻataʻitaʻiga.O sina vaega itiiti o mea leaga o lo'o mitiia e le lopa mageta e mafai ona aveese e le pa'u fufulu.I le TE, e mafai e le pulu mageta ona faʻasaʻo le DNA boundgenome, maua le DNA genome maualuga.O lenei metotia e faigofie ma vave ma o le DNA na maua mai e maualuga, lea e mafai ona ausia le manaʻoga mo le mauaina o le DNA methylation.I le taimi nei, o le pusa o loʻo faʻavae i luga o le maneta e mafai ona fetaui ma le faʻapipiʻiina o le nucleic acid otometi, faʻafeiloaʻi galuega faʻapipiʻi maualuga o le nucleic acid.
Vaega autu o le reagent
O vaega o loʻo faʻaalia i le laulau 1:
Fuafuaga 1 Reagent Vaega ma uta
| Igoa vaega | Vaega autu | Tele (48) | Tele (200) |
| 1. Fa'aliga Fa'aliga A | Tris, SDS | 15.8 mL/fagu | 66mL/fagu |
| 2. Lysis Buffer L | Guanidinium Isothiocyanate, Tris | 15.8 mL/fagu | 66mL/fagu |
| 3. Fufulu Fa'amau A | NaCl, Tris | 11 mL/fagu | 44 mL/fagu |
| 4. Fufulu Fa'amau B | NaCl, Tris | 13 mL/fagu | 26.5mL/fagu *2 |
| 5. TE | Tris, EDTA | 12 mL/fagu | 44 mL/fagu |
| 6. Fofo K Protease | Protease K | 1.1mL/ fasi | 4.4mL/ fasi |
| 7. Fa'agata maneta lopa 2 | Lopa maneta | 0.5mL/ fasi | 2.2mL/ fasi |
| 8. Fa'atonuga mo le su'eina ole nucleic acid reagents | / | 1 kopi | 1 kopi |
O vaega e mana'omia ile su'ega ole nucleic acid, ae le'o aofia ile pusa:
1. Reagent: Anhydrous ethanol, isopropanol, ma PBS;
2. Consumables: 50mL centrifuge tube ma 1.5mL EP paipa;
3. Meafaigaluega: Ta'ele vai, paipa , fata maneta, centrifuge, 96-well plate (otometi), masini fa'apipi'i otometi nucleic acid (otometi).
Fa'amatalaga autu
Fa'ata'ita'iga mana'oga:
1. E tatau ona maeʻa le suʻesuʻega i lalo o le 7-aso teuina o le vevela vevela pe a maeʻa le aoina o le faʻataʻitaʻiga o le cell exfoliated cervical (e le o faʻamautu).
2.O le suʻesuʻega e tatau ona maeʻa i lalo o le 30-aso teuina o le vevela vevela pe a maeʻa le aoina o le faʻataʻitaʻiga o le cell exfoliated cervical (faʻamau).
3. O le suʻesuʻega e tatau ona faʻamaeʻaina i lalo ole 30-aso teuina o le vevela vevela pe a maeʻa le aoina o le urine sample;Ole su'esu'ega e tatau ona fa'amae'a ile taimi pe a mae'a le aoina o fa'ata'ita'iga sela fa'aa'e.
Fa'amatalaga paka:200 pcs / pusa, 48 pcs / pusa.
Tulaga teuina:2-30 ℃
Vaitaimi o le aloaia:12 masina
Meafaigaluega talafeagai:Tianlong NP968-C nucleic acid extracting instrument, Tiangen TGuide S96 nucleic acid extracting instrument, GENE DIAN EB-1000 nucleic acid extracting instrument.
Numera fa'amaumauga o masini fa'afoma'i/mea e mana'omia fa'apitoa Nu.:HJXB Nu. 20210100.
Aso o le faʻatagaina ma le toe iloiloga o faʻatonuga:Aso fa'amaonia: Nov. 18, 2021
Faatatau ia tatou
I le avea ai o se atinaʻe faʻatekonolosi maualuga na faʻatūina i le 2018 e tagata tomai faʻapitoa epigenetic, Epiprobe e taulaʻi i le suʻesuʻega mole mole o le kanesa DNA methylation ma le faʻataʻitaʻiga saʻo.Faatasi ai ma se faavae faʻatekonolosi loloto, matou te faʻamoemoe e taʻitaʻia le vaitau o oloa fou e faʻaumatia le kanesa i le fuga!
Faʻavae i luga o suʻesuʻega umi a le Epiprobe core team, atinaʻe ma suiga i le fanua o le DNA methylation faʻatasi ai ma le faʻaogaina o mea fou, faʻatasi ai ma le DNA methylation faʻatatau o kanesa, matou te faʻaogaina se algorithm faʻapitoa multivariate e tuʻufaʻatasia ai faʻamatalaga tetele ma tekonolosi faʻapitoa e tuto'atasi e atia'e se faiga fa'atekonolosi vai e puipuia ai pateni.E ala i le suʻesuʻeina o le maualuga o le methylation o nofoaga faʻapitoa o vaevaega DNA saoloto i totonu o le faʻataʻitaʻiga, o faʻaletonu o metotia faʻaleaganuʻu masani ma tapulaʻa o taotoga ma faʻataʻitaʻiga puncture e aloese mai ai, e le gata ina maua ai le suʻesuʻeina saʻo o kanesa vave, ae mafai ai foi ona mataʻituina taimi moni. o le tupu mai o le kanesa ma le malosi o le atina'e.
