Gargle Nucleic Acid Extraction Reagents
FA'AMATALAGA FA'AVAE
O le oloa e masani ona aofia ai le gargle, sili ona-absorbant superparamagnetic nanospheres paluga, ma tulaga ese lysis reagents.O lopa mageta fa'apipi'i fa'apitoa e iai le so'otaga lelei mo vaega fa'aletino (e aofia ai fa'ama'i fua ma sela e a'afia i siama).Pe a faʻafesoʻotaʻi le vaifofo lysis, o le non-ionic cell / nucleus-membrane-breaking surfactants ma protease inhibitors i le vaifofo e mafai ona taofia le gaioiga o le DNA / RNA enzyme, ma faʻamautu ai le nucleic acid.O mea uma o le nucleic acid i vaega faʻaletino o le gargle e faʻamatuʻu lelei i le lysis solution, vave maua le nucleic acid.O fa'ata'ita'iga o le gargle o lo'o fa'aogaina ai lenei pusa e le mana'omia le fa'amamāina o le nucleic acid, lea e mafai ona fa'aoga sa'o ile pusa su'esu'e nucleic acid i lalo.
Vaega autu o le reagent
O vaega o loʻo faʻaalia i le laulau 1.
Siata 1 Vaega ma le utaina i totonu o pusa
| Igoa vaega | Vaega autu | Tele (1) | Tele (10) | Tele (30) | Tele (50) |
| 1. Taufu A | NaCl | 8mL/tu'u | 8mL/faaga *10 paipa | 8mL/faaga *30 paipa | 8mL/faaga *50 paipa |
| 2. A'oa'i Tulaga | PP | 1 fasi | 10 pcs | 30 pcs | 50 pcs |
| 3. Fofo Fa'atamaoaigaina B | Lopa maneta | 2mL/tu'u | 2mL/faaga *10 paipa | 2mL/faaga *30 paipa | 2mL/faaga *50 paipa |
| 4 Lysis Buffer C | Protease K | 0.2mL/ fasi | 0.2mL / fasi * 10 pcs | 0.2mL / fasi * 30 pcs | 0.2mL / fasi * 50 pcs |
| 5. Maneta Cap | Maneta | 1 fasi | 10 pcs | 30 pcs | 50 pcs |
O vaega e aoga mo le 12 masina.
O vaega e mana'omia ile su'ega ole nucleic acid, ae le'o aofia ile pusa:
1. Consumables: 1.5ml EP paipa;
2. Meafaigaluega: Ta'ele vai (po'o le ta'ele u'amea), paipa, ma centrifuge.
Fa'amatalaga autu
Fa'ata'ita'iga mana'oga:
1. Fa'atatau i le su'ega nucleic acid ma le fa'atamaoaigaina o fa'ata'ita'iga o le gargle.
2. E tatau ona fa'aopoopo le fa'ata'ita'iga o le gargle i le vaifofo fa'atamaoaigaina B i le taimi pe a uma le aoina.O lo'o maua mai maneta e tatau ona fa'afeiloa'i vave ile lysis buffer C.O faʻataʻitaʻiga faʻaopoopo i le lysis buffer C e mafai ona teuina i le vevela o le potu.
Fa'amatalaga fa'apipi'i: 1 fasi / pusa, 10 pcs / pusa, 30 pcs / pusa, ma 50 pcs / pusa.
Tulaga teuina: Ole fa'amaoaigaina fofo B ma lysis solution C e tatau ona teuina mo le 12 masina ile 2-8℃, ma isi vaega e mafai ona teuina mo le 12 masina i lalo ole r vevela potu;O le pusa e mafai ona feaveaʻi ma teuina mo sina taimi i lalo o le vevela, e le sili atu i le 5 aso.
Vaitaimi o le aloaia: 12 masina
Numera fa'amaumauga o masini fa'afoma'i/mea e mana'omia fa'apitoa Nu.:HJXB Nu. 20220086.
Aso o le fa'atagaina ma le toe iloiloga o Fa'atonuga:
Aso o le faamaoniga: Oketopa 26, 2022
