Pusa e Ave'esea ai Asi Nucleic (A01)
Fa'avae su'esu'ega
A maeʻa ona tuʻuina atu le DNA genomic e ala i le vaeluaina o sela ma le lysis buffer, e mafai e le pulu mageta ona filifili filifili i le DNA genomic i le faʻataʻitaʻiga.O sina vaega itiiti o mea leaga o lo'o mitiia e le lopa mageta e mafai ona aveese e le pa'u fufulu.I le TE, e mafai e le pulu mageta ona faʻasaʻo le DNA boundgenome, maua le DNA genome maualuga.O lenei metotia e faigofie ma vave ma o le DNA na maua mai e maualuga, lea e mafai ona ausia le manaʻoga mo le mauaina o le DNA methylation.I le taimi nei, o le pusa o loʻo faʻavae i luga o le maneta e mafai ona fetaui ma le faʻapipiʻiina o le nucleic acid otometi, faʻafeiloaʻi galuega faʻapipiʻi maualuga o le nucleic acid.
Vaega autu o le reagent
O vaega o loʻo faʻaalia i le laulau 1:
Fuafuaga 1 Reagent Vaega ma uta
| Igoa vaega | Vaega autu | Tele (48) | Tele (200) |
| 1. Lysis fofo | Guanidine hydrochloride, Tris | 11 mL/fagu | 44 mL/fagu |
| 2. Fa'amamā vaifofo A | NaCl, Tris | 11 mL/fagu | 44 mL/fagu |
| 3. Fa'amamā vaifofo B | NaCl, Tris | 13 mL/fagu | 26.5mL/fagu *2 |
| 4. Eluent | Tris, EDTA | 12 mL/fagu | 44 mL/fagu |
| 5. Fofo K Protease | Protease K | 1.1mL/ fasi | 4.4mL/ fasi |
| 6. Fa'agata maneta lopa 1 | Lopa maneta | 1.1mL/ fasi | 4.4mL/ fasi |
| 7. Fa'atonuga mo le su'eina ole nucleic acid reagents |
| 1 kopi | 1 kopi |
O vaega e mana'omia ile su'ega ole nucleic acid, ae le'o aofia ile pusa:
1. Reagent: Anhydrous ethanol, isopropanol ma PBS;
2. Consumables: 50ml centrifuge tube ma 1.5ml EP paipa;
3. Meafaigaluega: Kettle vai taele, pipettor, fata maneta, centrifuge, ipu loloto 96-pu (otometi), masini faʻapipiʻi otometi nucleic acid (otometi).
Fa'amatalaga autu
Fa'ata'ita'iga mana'oga
1. E tatau ona aloese mai le fa'afefe o le koluse i le va o fa'ata'ita'iga ile aoina ma le teuina o fa'ata'ita'iga.
2. E tatau ona maeʻa le suʻesuʻega i lalo o le 7-aso teuina o le vevela vevela pe a maeʻa le aoina o le faʻataʻitaʻiga o le cell exfoliated cervical (e le o faʻamau).O le su'esu'ega e tatau ona fa'amae'aina i lalo ole 30 aso e teu ai le vevela ole a'afia pe a mae'a le aoina o le urine fa'ata'ita'iga;Ole su'esu'ega e tatau ona fa'amae'a ile taimi pe a mae'a le aoina o fa'ata'ita'iga sela fa'aa'e.
Fa'amatalaga paka:200 pcs / pusa, 48 pcs / pusa.
Tulaga teuina:2-30 ℃
Vaitaimi o le aloaia:12 masina
Meafaigaluega talafeagai:Tianlong NP968-C nucleic acid extracting instrument, Tiangen TGuide S96 nucleic acid extracting instrument, GENE DIAN EB-1000 nucleic acid extracting instrument.
Numera fa'amaumauga o masini fa'afoma'i/mea e mana'omia fa'apitoa Nu.: HJXB Nu. 20210099.
Aso o le faʻatagaina ma le suiga o faʻatonuga:
Aso fa'amaonia: Nov. 18, 2021
